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As a structural basis for the transmembrane activation of the atrial natriuretic factor receptor, a site-specific crystallization of the receptor was performed. The ligand-receptor complex was produced in a stable form by a combination of limited proteolysis, fusion protein expression in Escherichia coli and receptor purification. A variety of conditions were tested for the formation of the ligand-receptor complex that were suitable for single-crystal X-ray analysis. After the optimization of the crystallization conditions, a [H-(N-Lys-Lys)-Gly]n synthetic peptide, in which the C-terminal Gly of the agonist is replaced with Lys, was crystallized in the presence of phosphatidylethanolamine, the lipid component of the natural atrial natriuretic factor receptor. Crystals of the ligand-receptor complex are monoclinic and contain three molecules per asymmetric unit. The three-dimensional structure was solved by the molecular replacement method, using the structure of the N-terminal portion of the atrial natriuretic factor receptor (amino acids 1-224) as a search model. The structure of a peptide containing the binding motif in the second extracellular loop [H-(N-Lys-Lys)-Gly]n reveals the structure of the active site, and a side-chain interaction between the first and second extracellular loops is identified. It provides new structural information about the mechanism of atrial natriuretic factor receptor activation.Abstract
The guest-host charge-transfer (GHC) luminescence in Eu
This is a file that has been uploaded by a user but it maybe a part of our database or of one of our partners or may be found on another website. The File Has been added by our users and we do not guarantee any of these facts. If you think that this is not a part of our database, please contact us by using contact button at the end of the file.This file is part of the “Songs Of My Soul” release date and new album tour dates announced.Â All albums and merchandise are now available at www.JustinAlbum.com. Â Â “Songs of My Soul” setlist and more Â Thank you for your support, Justin Bieber. NEW YORK, April 3, 2015 /PRNewswire/ — After launching its critically acclaimed “Justin Bieber Is Bieber”™ campaign, MyMusicRocks.com, the World’s largest online record store and music community, announced today the official 2015 “Justin Bieber Is Bieber” CD release event. Justin Bieber’s 15th studio album, “Justin Bieber Is Bieber” will be released by label Play Music in all countries. The Deluxe CD/DVD includes 14 tracks, a CD booklet of 31 photos, a lyric booklet and a DVD that features an exclusive concert on stage at the Teatro de la Marina in Havana, Cuba on February 23, 2015. Alongside the announcement of the new release, MyMusicRocks.com released its official “Justin Bieber Is Bieber” social media campaign. An exclusive 2 minute and 20 second video, directed by Rob Cohen, starts today on MyMusicRocks.com. The official TV commercial will appear on American television in the months to come. “The success of our ‘Justin Bieber Is Bieber’ campaign has exceeded our expectations. This was a very important project for us because it not only gave us the opportunity to celebrate the release of one of the most popular singer in the history of music,” said Carlos Tabo, MyMusicRocks CEO. “We had to play our role of ‘neutral’ record store to take the initiative and be different. This is why we chose to launch this campaign on MyMusicRocks.com, as this 37a470d65a
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